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KMID : 0379119860140010009
Korean Journal of Mycology
1986 Volume.14 No. 1 p.9 ~ p.15
Genetic Analysis of the Life Cycle in Interspecific Hybrids of Pleurotus ostreatus and Pleurotus florida Following Protoplast Fusion
¹Ú¿ëȯ/Park, Yong Hwan
À¯¿µº¹/À¯Ã¢Çö/ÁÔÆä¹öµð/Yoo, Young Bok/You, Chang Hyun/Peberdy, John F.
Abstract
Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
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